RAPID COMMUNICATION Syngeneic Transplantation With Peripheral Blood Mononuclear Cells Collected After the Administration of Recombinant Human Granulocyte Colony-Stimulating Factor

Five syngeneic transplants were performed in four patients following myeloablative therapy using unmodified peripheral blood mononuclear cells (PBMCs) collected after the administration of recombinant human granulocyte colony stimulating factor (rhG-CSF) to normal donors. The only toxicity experienced by the four normal donors was bone pain. Four patients received two collections of PBMCs, and a second transplant was performed in one patient with one collection. The patients received a median of 20.53 X 1 O8 total nucleated cells/kg (range 20 to 25.5). 1 1.3 X I O 8 total mononuclear cells/kg (range 6.52 to 17.2), 1 13.1 X 104/kg CFU-GM (range 46.7 to 211.8) and 9.6 X I O 6 CD34+ cells/kg (range 1.6 to 12.6) Post-transplant growth factors were not administered. The median time to an absolute neutrophil count greater than 0.5 X 109/L